Super resolution microscopy | Stimulated emission depletion (STED) microscopy

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Published at : 07 Jan 2021
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Stimulated emission depletion (STED) microscopy is one of the techniques that make up super-resolution microscopy. It creates super-resolution images by the selective deactivation of fluorophores, minimising the area of illumination at the focal point, and thus enhancing the achievable resolution for a given system.

It was developed by Stefan W. Hell and Jan Wichmann in 1994, and was first experimentally demonstrated by Hell and Thomas Klar in 1999. Hell was awarded the Nobel Prize in Chemistry in 2014 for its development.
stimulated emission depletion microscopyhow does sted microscopy worksted microscopy review

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